Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych

Societatea Romana de Stiinte Fiziologice  > ELISA kit, immune, Isotype, miRNA >  Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych
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Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych

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Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych limfocytów pacjenta ze skórnym chłoniakiem T-komórkowym.

 Particule de retrovirus cu morfologie de tip C au fost găsite în două  linii celulare  limfoblastoidiene de celule T  , HUT 102 și CTCL-3, și în limfocite proaspete de sânge periferic   obținute de la un pacient cu limfom cutanat cu celule  T (micoză fungoidă). Liniile  celulare  produc continuu aceste virusuri, care sunt denumite în mod colectiv HTLV, tulpina CR (HTLV (CR)).

Inițial, producția de virus din celulele HUT 102 a   necesitat inducerea cu 5-iodo-2′-deoxiuridină, dar   linia celulară a devenit un producător constitutiv de virus la pasajul 56.  Linia celulară CTCL-Three a fost un producător constitutiv de virus de la al doilea pasaj în cultură.

Ambele particule de virus ular celular additional matur și imatur au fost observate în micrografii electronice cu secțiune subțire de materials ular repair cu celule peletate  ; ocazional, s-au văzut particule tipice de virus în devenire de tip C. Nu s-a văzut nicio formă de particulă de virus ular intra celular .

Particulele mature aveau un diametru de 100-110 nm, constau dintr-un miez dens de electroni înconjurat de o membrană exterioară separată de o regiune electron-lucentă, bandată la o densitate de 1,16 g / ml pe un gradient continuu de zaharoză de 25-65% și conținea ARN 70S și o activitate ADN polimerază tipică pentru transcriptaza inversă virală (RT; ADN nucleotidiltransferază dependentă de ARN). În anumite condiții de testare, HTLV (CR) RT a arătat preferința cationică pentru Mg (2+) față de Mn (2+), distinctă de caracteristicile ADN polimeraze celulare ular purificate din limfocite umane  și RT din majoritatea virusurilor de tip C.

Anticorpii împotriva  ADN polimerazei ular celulare gamma și anticorpilor împotriva RT purificați din mai multe retrovirusuri animale nu au reușit să interacționeze detectabil cu HTLV (CR) RT în condiții pozitive pentru ADN polimeraza omologă respectivă, demonstrând lipsa unei relații strânse de HTLV ) RT la  ADN polimeraze celulare ular gamma sau RT ale acestor virusuri. Șase proteine ​​majore, cu dimensiuni de aproximativ 10.000, 13.000, 19.000, 24.000, 42.000 și 52.000 daltoni, au fost evidente atunci când particulele HTLV (CR) dublu bandate și perturbate au fost cromatografiate pe o NaDodSO (4) / poliacrilamidăgel. Numărul acestor proteine ​​asociate particulelor este în concordanță cu proteinele așteptate ale unui retrovirus, dar dimensiunile unora sunt diferite de cele ale celor mai cunoscute retrovirusuri ale subgrupurilor de primate.

Izolacja retrowirusa limfotropowego T od pacjenta z ryzykiem zespołu nabytego niedoboru odporności (AIDS).

Un retrovirus aparținând familiei  virusurilor leucemice cu celule T umane (HTLV) current descoperite , dar clar distinct de fiecare izolat anterior, a fost izolat de la un pacient caucazian cu semne și simptome care preced adesea sindromului imunodeficienței dobândite (SIDA). Acest virus este un virus tumoral tipic ARN tip C, muguri din   membrana celulară , preferă magneziul pentru activitatea de transcriptază inversă și are un antigen intern (p25) comparable cu HTLV p24.

Anticorpii din serul acestui pacient reacționează cu proteinele din virusurile subgrupului HTLV-I, dar antiserurile de tip particular la HTLV-I nu precipită proteinele noului izolat. Virusul de la acest pacient a fost transmis în limfocitele din sângele cordonului ombilical  , iar virusul produs de aceste  celule  este comparable cu izolatul unique. Din aceste studii se concluzionează că acest virus, precum și izolatele anterioare HTLV aparțin unei familii generale de retrovirusuri T-limfotrope care se transmit orizontal la om și pot fi implicate în mai multe sindroame patologice, inclusiv SIDA.

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Zaangażowanie imunoinhibitorowego receptora PD-1 przez nowego członka rodziny B7 prowadzi do negatywnej regulacji aktywacji limfocytów. 

PD-1 este un receptor imunoinhibitor exprimat de celule T activate  , celule B  și celule mieloide  . Șoarecii deficienți în PD-1 prezintă o defalcare a toleranței periferice și demonstrează a number of caracteristici autoimune. Raportăm aici că ligandul PD-1 (PD-L1) este un membru al familiei genelor B7. Angajarea PD-1 de către PD-L1 duce la inhibarea  proliferării limfocitelor  mediate de receptorul  celulelor T  și a secreției de citokine. În plus, semnalizarea PD-1 poate inhiba cel puțin niveluri suboptimale de costimulare mediată de CD28. PD-L1 este exprimat de celule care prezintă antigen  , inclusiv monocite de sânge periferic uman stimulate cu interferon gamma și dendritic uman și murin activat celule .

În plus, PD-L1 este exprimat în țesuturi nelimfoide, cum ar fi inima și plămânii. Nivelurile relative de inhibare a PD-L1 și costimulare B7-1 / B7-2 semnalelor pe prezentatoare de antigen  celule  poate determina gradul de T  cu celule de  activare și , prin urmare , pragul dintre toleranță și autoimunitate. Expresia PD-L1 pe țesuturile nelimfoide și interacțiunea sa potențială cu PD-1 pot determina ulterior amploarea răspunsurilor imune la locurile de inflamație.

U myszy z niedoborem interleukiny 10 rozwija się przewlekłe zapalenie jelit.

Interleukina-10 (IL-10) afectează creșterea și diferențierea multor celule hemopoietice   in vitro; în particular, este un puternic supresor al  funcțiilor macrofagelor și celulelor T. La șoarecii cu deficiență de IL-10, generați prin direcționarea genelor,   dezvoltarea limfocitelor și răspunsurile la anticorpi sunt normale, dar majoritatea animalelor sunt retardate în creștere și anemice și suferă de enterocolită cronică. Modificările intestinului includ hiperplazie mucoasă extinsă, reacții inflamatorii și expresia aberantă a moleculelor majore de complicated de histocompatibilitate clasa II pe epitelii.

În schimb, mutanții păstrați în condiții specifice fără patogeni dezvoltă doar o inflamație locală limitată la colonul proximal. Aceste rezultate indică faptul că inflamația intestinului la mutanți provine din răspunsuri imune necontrolate stimulate de antigeni enterici și că IL-10 este un imunoreglator esențial în tractul intestinal.

Prosta technika ilościowego określania niskich poziomów uszkodzeń DNA w poszczególnych komórkach.

Limfocitele umane   au fost fie expuse la iradiere X (25 până la 200 rads), fie tratate cu H2O2 (9,1 până la 291 microM) la Four grade C și gradul de migrare a ADN-ului a fost măsurat folosind o  tehnică de electroforeză cu microgel unicelular în condiții alcaline. Ambii agenți au indus o creștere semnificativă a migrației ADN, începând cu cea mai mică doză evaluată.

Modelele de migrație au fost relativ omogene între  celulele  expuse la raze X, dar eterogene între  celulele  tratate cu H2O2. O analiză a cineticii de reparație după expunerea la raze X de 200 rads a fost efectuată cu  limfocite  obținute de la trei persoane. Cea mai mare parte a reparației ADN a avut loc în primele 15 minute, în timp ce toată reparația a fost în esență completă la 120 de minute după expunere. Cu toate acestea, unele  celule  nu au demonstrat nicio reparație în această perioadă de incubație, în timp ce alte  celule au  demonstrat modele de migrare a ADN care indică mai multe daune decât cele induse de iradierea inițială cu raze X. Această tehnică pare a fi sensibilă și utilă pentru detectarea daunelor și repararea în celule unice .

Ccl2 CRISPR Knock Out CT26 Cell Line

T9611 1x10^6 cells / 1.0 ml Ask for price

CXCL8 CRISPR Knock Out L3.6pl Cell Line

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CXCL1 CRISPR Knock Out L3.6pl Cell Line

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FOLR CRISPR Knock Out Human OVCAR3-A1 Cell Line

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MUC16 CRISPR Knock Out Human OVCAR3-A1 Cell Line

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NR2F6 CRISPR Knock Out Human Jurkat E6.1 Cell Line - Clone T3-10

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TREM2 CRISPR Knock Out Human THP-1 Cell Line - Clone T1-9

T6810 1x10^6 cells / 1.0 ml Ask for price

PTPN6 CRISPR Knock Out Human THP-1 Cell Line - Clone T3-1

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Aldh2 CRISPR Knock Out L929 Cell Line - Clone T3-20

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PTPN11 CRISPR Knock Out NIH/3T3 Cell Line - Clone T4-9

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METTL3 CRISPR Knock Out Caki-1 Cell Line - Clone T2-16

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EUR 3950

Nacc1 CRISPR Knock Out ID8 Cell Line (Mouse) - Clone T1-23

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osbpl2 CRISPR Knock Out NIH/3T3 Cell Line - T1 Polyclonal Pool

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GLT1 CRISPR Knock Out Rat AST Cell Line - Clone T2-1

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PRSS21 CRISPR Knock Out Human OVCAR8 Cell Line - Clone T2 C8

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Aifm1 CRISPR Knock Out Mouse L929 Cell Line - Clone T2-15-3

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SEC61G CRISPR Knock Out HCC-LM3 Cell Line - Clone T2-17-5

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MSLN CRISPR Knock Out Human OVCAR3-A1 Cell Line - Clone T2 C4

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CARMIL1 CRISPR Knock Out Immortalized Human Gingival Fibroblast - hTERT Cell Line - T1-2

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hPIM1 CRISPR Knock Out SACC-83 Cell Line-T3-9

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Dcx CRISPR Knock Out C6 Cell Line (Rat) - Clone sg4-1

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Cell line gene knock-in and knock-out

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Human LY96 knockout cell line

ABC-KH8810 1 vial Ask for price
Description: Human LY96 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY96 knockdown cell line

ABC-KD8810 1 vial Ask for price
Description: Human LY96 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T1-19

T9511 1x10^6 cells / 1.0 ml
EUR 3950

SIRT1 Knockout Stable 293T Cell Line

T3176 1x10^6 cells / 1.0 ml
EUR 3950

Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T3-3

T9529 1x10^6 cells/1.0ml
EUR 3950

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line

T3187 1x10^6 cells / 1.0 ml
EUR 3950

293T Cell Line

LV010 1x10^6
EUR 450

293T [HEK-293T] Cell Line

CL-0005 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

293T/17 Cell Line

CL-0469 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

293T Cell Lysate (Human embryonic kidney cell line)

LF-R0006 200 ul
EUR 103.2
Description: 293T (Human embryonic kidney cell line) Whole Cell Lysate

293AD Cell Line

AD-100 1 vial
EUR 365

293AAV Cell Line

AAV-100 1 vial
EUR 405

293LTV Cell Line

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293RTV Cell Line

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293/GFP Cell Line

AKR-200 1 vial
EUR 460

Human LY75 knockout cell line

ABC-KH8806 1 vial Ask for price
Description: Human LY75 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY86 knockout cell line

ABC-KH8808 1 vial Ask for price
Description: Human LY86 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

A549/GFP Cell Line

AKR-209 1 vial
EUR 460

HeLa/GFP Cell Line

AKR-213 1 vial
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293/Cas9 Cell Line

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HeLa/Cas9 Cell Line

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EUR 460

NIH3T3/GFP Cell Line

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NIH3T3/Cas9 Cell Line

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Cas9 Expressing 293T Cell Line

T3251 1x10^6 cells / 1.0 ml
EUR 795

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Blank Promoter Stable Expressing Human 293T Cell Line

T9618 1x10^6 cells / 1.0 ml
EUR 3950

Human LY75 knockdown cell line

ABC-KD8806 1 vial Ask for price
Description: Human LY75 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY86 knockdown cell line

ABC-KD8808 1 vial Ask for price
Description: Human LY86 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Stable 293T-ATD cell line

T6340 1x10^6 cells / 1.0 ml
EUR 3950

Human LY75-CD302 knockout cell line

ABC-KH8807 1 vial Ask for price
Description: Human LY75-CD302 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Mouse NACC1 CRISPR Stable Knockout ID8 Cell Line - Clone T1-23

T6826 1x10^6 cells / 1.0 ml
EUR 3950

Human LY9 knockout cell line

ABC-KH8809 1 vial Ask for price
Description: Human LY9 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

PTPN6 Stable Knocked Out THP-1 Cell Line

T6216 1x10^6 cells / 1.0 ml
EUR 3950

Human LY6D knockout cell line

ABC-KH8797 1 vial Ask for price
Description: Human LY6D knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6E knockout cell line

ABC-KH8798 1 vial Ask for price
Description: Human LY6E knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6H knockout cell line

ABC-KH8804 1 vial Ask for price
Description: Human LY6H knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6K knockout cell line

ABC-KH8805 1 vial Ask for price
Description: Human LY6K knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G5B knockout cell line

ABC-KH8799 1 vial Ask for price
Description: Human LY6G5B knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G5C knockout cell line

ABC-KH8800 1 vial Ask for price
Description: Human LY6G5C knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G6C knockout cell line

ABC-KH8801 1 vial Ask for price
Description: Human LY6G6C knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G6D knockout cell line

ABC-KH8802 1 vial Ask for price
Description: Human LY6G6D knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G6F knockout cell line

ABC-KH8803 1 vial Ask for price
Description: Human LY6G6F knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

SIRT1 Wildtype Stable 293T Cell Line

T3175 1x10^6 cells / 1.0 ml
EUR 3950

Human LY75-CD302 knockdown cell line

ABC-KD8807 1 vial Ask for price
Description: Human LY75-CD302 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY9 knockdown cell line

ABC-KD8809 1 vial Ask for price
Description: Human LY9 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 770

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 770

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
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Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Human LY6D knockdown cell line

ABC-KD8797 1 vial Ask for price
Description: Human LY6D knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6E knockdown cell line

ABC-KD8798 1 vial Ask for price
Description: Human LY6E knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6H knockdown cell line

ABC-KD8804 1 vial Ask for price
Description: Human LY6H knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6K knockdown cell line

ABC-KD8805 1 vial Ask for price
Description: Human LY6K knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G5B knockdown cell line

ABC-KD8799 1 vial Ask for price
Description: Human LY6G5B knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G5C knockdown cell line

ABC-KD8800 1 vial Ask for price
Description: Human LY6G5C knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G6C knockdown cell line

ABC-KD8801 1 vial Ask for price
Description: Human LY6G6C knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G6D knockdown cell line

ABC-KD8802 1 vial Ask for price
Description: Human LY6G6D knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G6F knockdown cell line

ABC-KD8803 1 vial Ask for price
Description: Human LY6G6F knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

PGC-1α/ERRα Stable 293T Cell Line

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EUR 385

Mouse R-spondin1 Stable 293T Cell Line

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EUR 3950

BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line

T6245 1x10^6 cells / 1.0 ml
EUR 3950

PFN2 Stable Expressing 293T Cell Line - Polyclonal

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EUR 3950

PLN Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)

iPS41 1 Vial
EUR 2268

LRP1 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)

iPS31 1 Vial
EUR 2268

AFF2 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)

iPS61 1 Vial
EUR 2268

ABCA7 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)

iPS21 1 Vial
EUR 2268

KCNK3 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)

iPS51 1 Vial
EUR 2268

LLGL1 Knock-out Human Induced Pluripotent Stem Cells (Episomal, HFF)

iPS71 1 Vial
EUR 2268

TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line)

AST-7001 1 Kit Ask for price
Description: 6 month

Human CRISP1 knockout cell line

ABC-KH3569 1 vial Ask for price
Description: Human CRISP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISP2 knockout cell line

ABC-KH3570 1 vial Ask for price
Description: Human CRISP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISP3 knockout cell line

ABC-KH3571 1 vial Ask for price
Description: Human CRISP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

PTPRA CRISPR Knockout Immortalized Human Lung Fibroblast Cell Line

T6827 1x10^6 cells / 1.0 ml Ask for price

YBX1 Stable Knockout Human NK/T Cell Line #T3-16 P6

T9508 1x10^6 cells / 1.0 ml Ask for price

Human CRISPLD1 knockout cell line

ABC-KH3572 1 vial Ask for price
Description: Human CRISPLD1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISPLD2 knockout cell line

ABC-KH3573 1 vial Ask for price
Description: Human CRISPLD2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

TARGATT? Knock-in iPSC Generation (Master Cell Line)

AST-1100 1 vial of 1X10^6 cells Ask for price
Description: 6 month

Human 293T Cell Lysate Lyophilized

IHU293TTLLY200UG each
EUR 89
Description: Human 293T Cell Lysate Lyophilized

Acan CRISPR Knockout RCS Cell Line (Rat)

T3000 1x10^6 cells / 1.0 ml Ask for price

LY96 3'UTR GFP Stable Cell Line

TU062782 1.0 ml
EUR 1672.8

Ly96 3'UTR GFP Stable Cell Line

TU162731 1.0 ml Ask for price

Ly96 3'UTR GFP Stable Cell Line

TU262699 1.0 ml Ask for price

Human CRISP1 knockdown cell line

ABC-KD3569 1 vial Ask for price
Description: Human CRISP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISP2 knockdown cell line

ABC-KD3570 1 vial Ask for price
Description: Human CRISP2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISP3 knockdown cell line

ABC-KD3571 1 vial Ask for price
Description: Human CRISP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human 293T Whole Cell Lysate

LYSATE0032 200ug
EUR 180
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

LY96 3'UTR Luciferase Stable Cell Line

TU012782 1.0 ml
EUR 1672.8

Ly96 3'UTR Luciferase Stable Cell Line

TU112731 1.0 ml Ask for price

Ly96 3'UTR Luciferase Stable Cell Line

TU212699 1.0 ml Ask for price

Human CRISPLD1 knockdown cell line

ABC-KD3572 1 vial Ask for price
Description: Human CRISPLD1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISPLD2 knockdown cell line

ABC-KD3573 1 vial Ask for price
Description: Human CRISPLD2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)

AST-1200 1 Kit Ask for price
Description: 12 month

VEGF Promoter Stable Expressing 293T Cell Line - Clone #2

T9622 1x10^6 cells / 1.0 ml
EUR 3950

AAVS1 Positive Control EGIP 293T Reporter Cell Line

CAS606A-1 1 Vial
EUR 726

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)

AST-1300 1 Kit Ask for price
Description: 12 month

GFAT1 CRISPR Stable Knockout H1299 Cell Line

T6199 1x10^6 cells / 1.0 ml
EUR 3950

EDC4 CRISPR Stable Knockout HEK293T Cell Line

T6241 1x10^6 cells / 1.0 ml
EUR 3950

293T Cell Lysates

GWB-E5C7FC 0.2 mg Ask for price

DJ-1 CRISPR Stable Knockout HeLa Cell Line

T6181 1x10^6 cells / 1.0 ml
EUR 3950

PDPN CRISPR Stable Knockout COS-7 Cell Line

T6039 1x10^6 cells / 1.0 ml
EUR 3950

PD-L1 CRISPR Stable Knockout CT26 Cell Line

T6237 1x10^6 cells / 1.0 ml
EUR 3950

PD-L1 CRISPR Stable Knockout MC38 Cell Line

T6149 1x10^6 cells / 1.0 ml
EUR 3950

LIMD1-/- CRISPR Stable Knockout HeLa Cell Line

T6357 1x10^6 cells / 1.0 ml
EUR 3950

SLC35A1 CRISPR Stable Knockout CHO-S Cell Line

T6040 1x10^6 cells / 1.0 ml
EUR 3950

CXCL1 & CXCL8 CRISPR Double Knockout L3.6pl Cell Line

T6047 1x10^6 cells / 1.0 ml Ask for price

AXL CRISPR Stable Knockout HT1080 (1-IV) Cell Line

T6232 1x10^6 cells / 1.0 ml
EUR 3950

AXL CRISPR Stable Knockout HT1080 (3-II) Cell Line

T6233 1x10^6 cells / 1.0 ml
EUR 3950

LY96 sgRNA CRISPR Lentivector set (Human)

K1246301 3 x 1.0 ug
EUR 406.8

hCETP shRNA (Target C) Knock Down THP-1 Cell Line-Polyclone

T9789 1x10^6 cells / 1.0 ml Ask for price

Human CRIM1 knockout cell line

ABC-KH3563 1 vial Ask for price
Description: Human CRIM1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP1 knockout cell line

ABC-KH3564 1 vial Ask for price
Description: Human CRIP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP2 knockout cell line

ABC-KH3565 1 vial Ask for price
Description: Human CRIP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP3 knockout cell line

ABC-KH3566 1 vial Ask for price
Description: Human CRIP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIPT knockout cell line

ABC-KH3568 1 vial Ask for price
Description: Human CRIPT knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIPAK knockout cell line

ABC-KH3567 1 vial Ask for price
Description: Human CRIPAK knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

LY96 sgRNA CRISPR Lentivector (Human) (Target 1)

K1246302 1.0 ug DNA
EUR 184.8

LY96 sgRNA CRISPR Lentivector (Human) (Target 2)

K1246303 1.0 ug DNA
EUR 184.8

LY96 sgRNA CRISPR Lentivector (Human) (Target 3)

K1246304 1.0 ug DNA
EUR 184.8

Cas9 (CRISPR Associated Protein 9) ELISA Kit

PRB-5079 96 assays
EUR 485

Human CRIM1 knockdown cell line

ABC-KD3563 1 vial Ask for price
Description: Human CRIM1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIP1 knockdown cell line

ABC-KD3564 1 vial Ask for price
Description: Human CRIP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIP2 knockdown cell line

ABC-KD3565 1 vial Ask for price
Description: Human CRIP2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIP3 knockdown cell line

ABC-KD3566 1 vial Ask for price
Description: Human CRIP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIPT knockdown cell line

ABC-KD3568 1 vial Ask for price
Description: Human CRIPT knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIPAK knockdown cell line

ABC-KD3567 1 vial Ask for price
Description: Human CRIPAK knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LIN37 knockout cell line

ABC-KH8476 1 vial Ask for price
Description: Human LIN37 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human GC knockout cell line

ABC-KH5948 1 vial Ask for price
Description: Human GC knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human GK knockout cell line

ABC-KH6087 1 vial Ask for price
Description: Human GK knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human HP knockout cell line

ABC-KH6972 1 vial Ask for price
Description: Human HP knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human HR knockout cell line

ABC-KH6992 1 vial Ask for price
Description: Human HR knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human KL knockout cell line

ABC-KH7942 1 vial Ask for price
Description: Human KL knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human KY knockout cell line

ABC-KH8250 1 vial Ask for price
Description: Human KY knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human MB knockout cell line

ABC-KH9065 1 vial Ask for price
Description: Human MB knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F2 knockout cell line

ABC-KH5009 1 vial Ask for price
Description: Human F2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F3 knockout cell line

ABC-KH5014 1 vial Ask for price
Description: Human F3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

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