Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych

Societatea Romana de Stiinte Fiziologice  > ELISA kit, immune, Isotype, miRNA >  Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych
srsf

Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych

0 Comments

Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych limfocytów pacjenta ze skórnym chłoniakiem T-komórkowym.

 Particule de retrovirus cu morfologie de tip C au fost găsite în două  linii celulare  limfoblastoidiene de celule T  , HUT 102 și CTCL-3, și în limfocite proaspete de sânge periferic   obținute de la un pacient cu limfom cutanat cu celule  T (micoză fungoidă). Liniile  celulare  produc continuu aceste virusuri, care sunt denumite în mod colectiv HTLV, tulpina CR (HTLV (CR)).

Inițial, producția de virus din celulele HUT 102 a   necesitat inducerea cu 5-iodo-2′-deoxiuridină, dar   linia celulară a devenit un producător constitutiv de virus la pasajul 56.  Linia celulară CTCL-Three a fost un producător constitutiv de virus de la al doilea pasaj în cultură.

Ambele particule de virus ular celular additional matur și imatur au fost observate în micrografii electronice cu secțiune subțire de materials ular repair cu celule peletate  ; ocazional, s-au văzut particule tipice de virus în devenire de tip C. Nu s-a văzut nicio formă de particulă de virus ular intra celular .

Particulele mature aveau un diametru de 100-110 nm, constau dintr-un miez dens de electroni înconjurat de o membrană exterioară separată de o regiune electron-lucentă, bandată la o densitate de 1,16 g / ml pe un gradient continuu de zaharoză de 25-65% și conținea ARN 70S și o activitate ADN polimerază tipică pentru transcriptaza inversă virală (RT; ADN nucleotidiltransferază dependentă de ARN). În anumite condiții de testare, HTLV (CR) RT a arătat preferința cationică pentru Mg (2+) față de Mn (2+), distinctă de caracteristicile ADN polimeraze celulare ular purificate din limfocite umane  și RT din majoritatea virusurilor de tip C.

Anticorpii împotriva  ADN polimerazei ular celulare gamma și anticorpilor împotriva RT purificați din mai multe retrovirusuri animale nu au reușit să interacționeze detectabil cu HTLV (CR) RT în condiții pozitive pentru ADN polimeraza omologă respectivă, demonstrând lipsa unei relații strânse de HTLV ) RT la  ADN polimeraze celulare ular gamma sau RT ale acestor virusuri. Șase proteine ​​majore, cu dimensiuni de aproximativ 10.000, 13.000, 19.000, 24.000, 42.000 și 52.000 daltoni, au fost evidente atunci când particulele HTLV (CR) dublu bandate și perturbate au fost cromatografiate pe o NaDodSO (4) / poliacrilamidăgel. Numărul acestor proteine ​​asociate particulelor este în concordanță cu proteinele așteptate ale unui retrovirus, dar dimensiunile unora sunt diferite de cele ale celor mai cunoscute retrovirusuri ale subgrupurilor de primate.

Izolacja retrowirusa limfotropowego T od pacjenta z ryzykiem zespołu nabytego niedoboru odporności (AIDS).

Un retrovirus aparținând familiei  virusurilor leucemice cu celule T umane (HTLV) current descoperite , dar clar distinct de fiecare izolat anterior, a fost izolat de la un pacient caucazian cu semne și simptome care preced adesea sindromului imunodeficienței dobândite (SIDA). Acest virus este un virus tumoral tipic ARN tip C, muguri din   membrana celulară , preferă magneziul pentru activitatea de transcriptază inversă și are un antigen intern (p25) comparable cu HTLV p24.

Anticorpii din serul acestui pacient reacționează cu proteinele din virusurile subgrupului HTLV-I, dar antiserurile de tip particular la HTLV-I nu precipită proteinele noului izolat. Virusul de la acest pacient a fost transmis în limfocitele din sângele cordonului ombilical  , iar virusul produs de aceste  celule  este comparable cu izolatul unique. Din aceste studii se concluzionează că acest virus, precum și izolatele anterioare HTLV aparțin unei familii generale de retrovirusuri T-limfotrope care se transmit orizontal la om și pot fi implicate în mai multe sindroame patologice, inclusiv SIDA.

srsf
srsf

Zaangażowanie imunoinhibitorowego receptora PD-1 przez nowego członka rodziny B7 prowadzi do negatywnej regulacji aktywacji limfocytów. 

PD-1 este un receptor imunoinhibitor exprimat de celule T activate  , celule B  și celule mieloide  . Șoarecii deficienți în PD-1 prezintă o defalcare a toleranței periferice și demonstrează a number of caracteristici autoimune. Raportăm aici că ligandul PD-1 (PD-L1) este un membru al familiei genelor B7. Angajarea PD-1 de către PD-L1 duce la inhibarea  proliferării limfocitelor  mediate de receptorul  celulelor T  și a secreției de citokine. În plus, semnalizarea PD-1 poate inhiba cel puțin niveluri suboptimale de costimulare mediată de CD28. PD-L1 este exprimat de celule care prezintă antigen  , inclusiv monocite de sânge periferic uman stimulate cu interferon gamma și dendritic uman și murin activat celule .

În plus, PD-L1 este exprimat în țesuturi nelimfoide, cum ar fi inima și plămânii. Nivelurile relative de inhibare a PD-L1 și costimulare B7-1 / B7-2 semnalelor pe prezentatoare de antigen  celule  poate determina gradul de T  cu celule de  activare și , prin urmare , pragul dintre toleranță și autoimunitate. Expresia PD-L1 pe țesuturile nelimfoide și interacțiunea sa potențială cu PD-1 pot determina ulterior amploarea răspunsurilor imune la locurile de inflamație.

U myszy z niedoborem interleukiny 10 rozwija się przewlekłe zapalenie jelit.

Interleukina-10 (IL-10) afectează creșterea și diferențierea multor celule hemopoietice   in vitro; în particular, este un puternic supresor al  funcțiilor macrofagelor și celulelor T. La șoarecii cu deficiență de IL-10, generați prin direcționarea genelor,   dezvoltarea limfocitelor și răspunsurile la anticorpi sunt normale, dar majoritatea animalelor sunt retardate în creștere și anemice și suferă de enterocolită cronică. Modificările intestinului includ hiperplazie mucoasă extinsă, reacții inflamatorii și expresia aberantă a moleculelor majore de complicated de histocompatibilitate clasa II pe epitelii.

În schimb, mutanții păstrați în condiții specifice fără patogeni dezvoltă doar o inflamație locală limitată la colonul proximal. Aceste rezultate indică faptul că inflamația intestinului la mutanți provine din răspunsuri imune necontrolate stimulate de antigeni enterici și că IL-10 este un imunoreglator esențial în tractul intestinal.

Prosta technika ilościowego określania niskich poziomów uszkodzeń DNA w poszczególnych komórkach.

Limfocitele umane   au fost fie expuse la iradiere X (25 până la 200 rads), fie tratate cu H2O2 (9,1 până la 291 microM) la Four grade C și gradul de migrare a ADN-ului a fost măsurat folosind o  tehnică de electroforeză cu microgel unicelular în condiții alcaline. Ambii agenți au indus o creștere semnificativă a migrației ADN, începând cu cea mai mică doză evaluată.

Modelele de migrație au fost relativ omogene între  celulele  expuse la raze X, dar eterogene între  celulele  tratate cu H2O2. O analiză a cineticii de reparație după expunerea la raze X de 200 rads a fost efectuată cu  limfocite  obținute de la trei persoane. Cea mai mare parte a reparației ADN a avut loc în primele 15 minute, în timp ce toată reparația a fost în esență completă la 120 de minute după expunere. Cu toate acestea, unele  celule  nu au demonstrat nicio reparație în această perioadă de incubație, în timp ce alte  celule au  demonstrat modele de migrare a ADN care indică mai multe daune decât cele induse de iradierea inițială cu raze X. Această tehnică pare a fi sensibilă și utilă pentru detectarea daunelor și repararea în celule unice .

AAVS1 Positive Control EGIP 293T Reporter Cell Line

CAS606A-1 1 Vial
EUR 968.4

HEK-293T cells

T0011002 One Frozen vial
EUR 546

Gene Knock-Out HR Targeting Vector [MCS1-EF1?-RFP-T2A-Puro-pA-MCS2]

HR110PA-1 10 ug
EUR 1227.6

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]

HR410PA-1 10 ug
EUR 1227.6

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Hygro-pA-MCS2]

HR510PA-1 10 ug
EUR 1227.6

TARGATT? Knock-in iPSC Generation (Master Cell Line)

AST-1100 1 vial of 1X10^6 cells Ask for price
Description: 6 month

TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line)

AST-7001 1 Kit Ask for price
Description: 6 month

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)

AST-1200 1 Kit Ask for price
Description: 12 month

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)

AST-1300 1 Kit Ask for price
Description: 12 month

HEK-293T Telomerase Over-Expressing Cell Pellet

abx069991-1Pellet 1 Pellet
EUR 477.6

Ly96 3'UTR Luciferase Stable Cell Line

TU212699 1.0 ml Ask for price

Ly96 3'UTR GFP Stable Cell Line

TU262699 1.0 ml Ask for price

Ly96 3'UTR Luciferase Stable Cell Line

TU112731 1.0 ml Ask for price

Ly96 3'UTR GFP Stable Cell Line

TU162731 1.0 ml Ask for price

LY96 3'UTR GFP Stable Cell Line

TU062782 1.0 ml
EUR 1672.8

LY96 3'UTR Luciferase Stable Cell Line

TU012782 1.0 ml
EUR 1672.8

Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out

HR100PA-1 10 ug
EUR 1125.6

293T Transfection Kit (1 mL)

P902 - Ask for price

293T Transfection Kit (0.2 mL)

P902S - Ask for price

293T Transfection Kit (1 mL)

P903 - Ask for price

293T Transfection Kit (0.2 mL)

P903S - Ask for price

LY96 sgRNA CRISPR Lentivector set (Human)

K1246301 3 x 1.0 ug
EUR 406.8

LY96 Antibody

1-CSB-PA049918
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against LY96. Recognizes LY96 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:500-1:2000, IHC:1:25-1:100

LY96 Antibody

DF6669 200ul
EUR 420

LY96 Antibody

1-CSB-PA013254ESR1HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LY96. Recognizes LY96 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

LY96 Antibody

1-CSB-PA013254GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against LY96. Recognizes LY96 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

LY96 Antibody

1-CSB-PA013254LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LY96. Recognizes LY96 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

LY96 Antibody

32480-100ul 100ul
EUR 302.4

LY96 antibody

70R-10270 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal LY96 antibody

LY96 antibody

70R-18335 50 ul
EUR 522
Description: Rabbit polyclonal LY96 antibody

LY96 Antibody

ABD6669 100 ug
EUR 525.6

LY96 siRNA

20-abx923156
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

LY96 siRNA

20-abx923157
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Human LY96 shRNA Plasmid

20-abx958417
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

LY96 ELISA KIT|Human

EF010760 96 Tests
EUR 826.8

LY96 Recombinant Protein (Human)

RP018451 100 ug Ask for price

Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration

HR720PA-1 10 µg
EUR 1374

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

LY96 sgRNA CRISPR Lentivector (Human) (Target 1)

K1246302 1.0 ug DNA
EUR 184.8

LY96 sgRNA CRISPR Lentivector (Human) (Target 2)

K1246303 1.0 ug DNA
EUR 184.8

LY96 sgRNA CRISPR Lentivector (Human) (Target 3)

K1246304 1.0 ug DNA
EUR 184.8

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration

HR700PA-1 10 µg
EUR 1374

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration

HR710PA-1 10 µg
EUR 1374

TARGATT? Knock-in iPSC Genotyping Kit

AST-1102 1 Kit Ask for price
Description: 12 month

LY96 Blocking Peptide

DF6669-BP 1mg
EUR 234

LY96 Conjugated Antibody

C32480 100ul
EUR 476.4

LY96 cloning plasmid

CSB-CL013254HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the LY96 gene.

anti- LY96 antibody

FNab04900 100µg
EUR 702
Description: Antibody raised against LY96

LY96 Polyclonal Antibody

ES11009-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against LY96 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

LY96 Polyclonal Antibody

ES11009-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against LY96 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

LY96 Blocking Peptide

33R-6768 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of LY96 antibody, catalog no. 70R-10270

LY96 Polyclonal Antibody

ABP59169-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of LY96 from Human, Mouse. This LY96 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LY96 protein

LY96 Polyclonal Antibody

ABP59169-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of LY96 from Human, Mouse. This LY96 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LY96 protein

LY96 Polyclonal Antibody

ABP59169-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of LY96 from Human, Mouse. This LY96 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LY96 protein

LY96 Rabbit pAb

A1866-100ul 100 ul
EUR 369.6

LY96 Rabbit pAb

A1866-200ul 200 ul
EUR 550.8

LY96 Rabbit pAb

A1866-20ul 20 ul
EUR 219.6

LY96 Rabbit pAb

A1866-50ul 50 ul
EUR 267.6

Anti-LY96 antibody

PAab04900 100 ug
EUR 494.4

pcDNA3.1-LY96 Plasmid

PVTB00381-2a 2 ug
EUR 427.2

Anti-LY96 antibody

STJ24433 100 µl
EUR 332.4
Description: This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms.

Anti-LY96 antibody

STJ192167 200 µl
EUR 236.4
Description: Unconjugated Rabbit polyclonal to LY96

LY96 Polyclonal Antibody

A72050-050 50 ul
EUR 302.5

LY96 Polyclonal Antibody

A72050-100 100 ul
EUR 423.5

LY96 Polyclonal Antibody

A59694-020 20 ul
EUR 117.7

LY96 Polyclonal Antibody

A59694-050 50 ul
EUR 302.5

LY96 Polyclonal Antibody

A59694-100 100 ul
EUR 423.5

LY96 Polyclonal Antibody

A59694
  • EUR 684.66
  • EUR 117.70
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

LY96 Polyclonal Antibody

A72050
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

Human Lymphocyte antigen 96 (LY96)

1-CSB-EP013254HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Lymphocyte antigen 96(LY96),partial expressed in E.coli

LY96 ELISA Kit (Human) (OKCA01342)

OKCA01342 96 Wells
EUR 1015.2
Description: Description of target: Binds bacterial lipopolysaccharide (LPS). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sanadwich ELISA;Sensitivity: 31.25 pg/mL

LY96 ELISA Kit (Human) (OKAN05795)

OKAN05795 96 Wells
EUR 950.4
Description: Description of target: This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.062 ng/mL

LY96 ELISA Kit (Human) (OKCD00545)

OKCD00545 96 Wells
EUR 997.2
Description: Description of target: Binds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030). Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581). Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581).5 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.1"MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4."_x005F_x005F_x000D_Shimazu R., Akashi S., Ogata H., Nagai Y., Fukudome K., Miyake K., Kimoto M._x005F_x005F_x000D_J. Exp. Med. 189:1777-1782(1999) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLY-56, FUNCTION, INTERACTION WITH TLR4, SUBCELLULAR LOCATION.Ref.8"MD-2 enables Toll-like receptor 2 (TLR2)-mediated responses to lipopolysaccharide and enhances TLR2-mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components."_x005F_x005F_x000D_Dziarski R., Wang Q., Miyake K., Kirschning C.J., Gupta D._x005F_x005F_x000D_J. Immunol. 166:1938-1944(2001) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH TLR2 AND TLR4, FUNCTION.Ref.9"Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4."_x005F_x005F_x000D_Visintin A., Mazzoni A., Spitzer J.A., Segal D.M._x005F_x005F_x000D_Proc. Natl. Acad. Sci. U.S.A. 98:12156-12161(2001) [PubMed] [Europe PMC] [Abstract]Cited for: DISULFIDE BONDS, GLYCOSYLATION, SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH TLR4.Ref.11"Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran."_x005F_x005F_x000D_Kim H.M., Park B.S., Kim J.-I., Kim S.E., Lee J., Oh S.C., Enkhbayar P., Matsushima N., Lee H., Yoo O.J., Lee J.-O._x005F_x005F_x000D_Cell 130:906-917(2007) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 19-158 IN COMPLEX WITH TLR4 AND LIPOPOLYSACCHARIDE ANALOG, SUBUNIT.Ref.12"Crystal structures of human MD-2 and its complex with antiendotoxic lipid IVa."_x005F_x005F_x000D_Ohto U., Fukase K., Miyake K., Satow Y._x005F_x005F_x000D_Science 316:1632-1634(2007) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 17-160 IN COMPLEX WITH LIPID IV-A, DISULFIDE BONDS, GLYCOSYLATION AT ASN-26 AND ASN-114. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.062 ng/mL

LY96 ORF Vector (Human) (pORF)

ORF006151 1.0 ug DNA
EUR 114

LY96 ELISA Kit (Human) (OKDD00383)

OKDD00383 96 Wells
EUR 1028.4
Description: Description of target: This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms.;Species reactivity: Human;Application: ;Assay info: Quantitative Sandwich ELISA;Sensitivity: < 0.06 ng/mL

Microplate Swing-Out Centrifuge

abx725026-1Unit 1 Unit
EUR 1771.2

Clinical Swing-Out Centrifuge

abx725027-1Unit 1 Unit
EUR 1771.2

Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1?-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2]

HR210PA-1 10 ug
EUR 1374

Ly96 sgRNA CRISPR Lentivector set (Rat)

K6749501 3 x 1.0 ug
EUR 406.8

Ly96 sgRNA CRISPR Lentivector set (Mouse)

K4440001 3 x 1.0 ug
EUR 406.8

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

TARGATT? Knock-in iPSC Quick Knockin Kit

AST-1101 1 Kit Ask for price
Description: 12 month

Mouse LY96 shRNA Plasmid

20-abx971392
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

LY96 Antibody, HRP conjugated

1-CSB-PA013254LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LY96. Recognizes LY96 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

LY96 Antibody, FITC conjugated

1-CSB-PA013254LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LY96. Recognizes LY96 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

LY96 Antibody, Biotin conjugated

1-CSB-PA013254LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LY96. Recognizes LY96 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

LY96 Recombinant Protein (Rat)

RP210383 100 ug Ask for price

LY96 Recombinant Protein (Mouse)

RP148730 100 ug Ask for price

LY96 Recombinant Protein (Mouse)

RP148733 100 ug Ask for price

Human Lymphocyte Antigen 96 (LY96) Protein

20-abx067843
  • EUR 744.00
  • EUR 326.40
  • EUR 2230.80
  • EUR 878.40
  • EUR 543.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion (with PinPoint site already placed)

PIN320A-1 >2x10^5 cells
EUR 3724.8

Swing-out rotor 6-place

CEN0179 EACH
EUR 607.62

Swing-out rotor 8-place

CEN0180 EACH
EUR 701.1

Swing-out rotor 12-place

CEN0181 EACH
EUR 531.24

Rotor Swing out 6x5ml RB

CEN2634 EACH
EUR 429.78

Rotor Swing out 2x3MTP ID

CEN2664 EACH
EUR 1299.6

Autoclave Print-Out PAPER CR70

SXP374 EACH
EUR 40.7

Ly96 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6749502 1.0 ug DNA
EUR 184.8

Ly96 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6749503 1.0 ug DNA
EUR 184.8

Ly96 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6749504 1.0 ug DNA
EUR 184.8

Ly96 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4440002 1.0 ug DNA
EUR 184.8

Ly96 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4440003 1.0 ug DNA
EUR 184.8

Ly96 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4440004 1.0 ug DNA
EUR 184.8

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

LY96 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1246305 3 x 1.0 ug
EUR 451.2

Human Lymphocyte antigen 96(LY96) ELISA kit

CSB-EL013254HU-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Lymphocyte antigen 96 (LY96) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Lymphocyte antigen 96(LY96) ELISA kit

1-CSB-EL013254HU
  • EUR 843.60
  • EUR 5811.60
  • EUR 3084.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Lymphocyte antigen 96(LY96) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Lymphocyte Antigen 96 (LY96) ELISA Kit

20-abx152248
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Lymphocyte Antigen 96 (LY96) CLIA Kit

20-abx495523
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Lymphocyte antigen 96, LY96 ELISA KIT

ELI-14056h 96 Tests
EUR 988.8

LY96 Protein Vector (Human) (pPB-C-His)

PV024601 500 ng
EUR 394.8

LY96 Protein Vector (Human) (pPB-N-His)

PV024602 500 ng
EUR 394.8

LY96 Protein Vector (Human) (pPM-C-HA)

PV024603 500 ng
EUR 394.8

LY96 Protein Vector (Human) (pPM-C-His)

PV024604 500 ng
EUR 394.8

Lymphocyte Antigen 96 (LY96) Polyclonal Antibody (Human)

4-PAH705Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Lymphocyte Antigen 96 (LY96)

Human Lymphocyte Antigen 96(LY96)ELISA Kit

QY-E02325 96T
EUR 433.2

Human Lymphocyte Antigen 96 (LY96) ELISA Kit

SEH705Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Lymphocyte Antigen 96 (LY96) in tissue homogenates, cell lysates and other biological fluids.

Human Lymphocyte Antigen 96 (LY96) ELISA Kit

SEH705Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Lymphocyte Antigen 96 (LY96) in tissue homogenates, cell lysates and other biological fluids.

Human Lymphocyte Antigen 96 (LY96) ELISA Kit

SEH705Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Lymphocyte Antigen 96 (LY96) in tissue homogenates, cell lysates and other biological fluids.

Human Lymphocyte Antigen 96 (LY96) ELISA Kit

SEH705Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Lymphocyte Antigen 96 (LY96) in tissue homogenates, cell lysates and other biological fluids.

Human Lymphocyte Antigen 96 (LY96) ELISA Kit

4-SEH705Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Lymphocyte Antigen 96 (LY96) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Lymphocyte Antigen 96 ELISA Kit (LY96)

RK01810 96 Tests
EUR 625.2

Mouse Lymphocyte antigen 96 (Ly96)

1-CSB-MP013254MO
  • EUR 351.60
  • EUR 1155.60
  • EUR 490.80
  • EUR 860.40
  • 100ug
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Mouse Lymphocyte antigen 96(Ly96) expressed in Mammalian cell

Mouse Lymphocyte antigen 96 (Ly96)

1-CSB-EP013254MO
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Lymphocyte antigen 96(Ly96) expressed in E.coli

Lymphocyte Antigen 96 (LY96) Antibody

20-abx102165
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Lymphocyte Antigen 96 (LY96) Antibody

20-abx102166
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Lymphocyte Antigen 96 (LY96) Antibody

20-abx173395
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Lymphocyte Antigen 96 (LY96) Antibody

20-abx113544
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Lymphocyte Antigen 96 (LY96) Antibody

20-abx001540
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Lymphocyte Antigen 96 (LY96) Antibody

abx029509-400ul 400 ul
EUR 627.6

Lymphocyte Antigen 96 (LY96) Antibody

abx029509-80l 80 µl
EUR 343.2

Lymphocyte Antigen 96 (LY96) Antibody

20-abx212140
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Lymphocyte Antigen 96 (LY96) Antibody

20-abx225272
  • EUR 444.00
  • EUR 727.20
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 50 ul

Lymphocyte Antigen 96 (LY96) Antibody

20-abx320139
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

Lymphocyte Antigen 96 (LY96) Antibody

20-abx304411
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Lymphocyte Antigen 96 (LY96) Antibody

abx234900-100ug 100 ug
EUR 661.2

Polyclonal LY96 Antibody (C-term)

APR17258G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LY96 (C-term). This antibody is tested and proven to work in the following applications:

LY96 ELISA Kit (Mouse) (OKCA01713)

OKCA01713 96 Wells
EUR 1015.2
Description: Description of target: Binds bacterial lipopolysaccharide (LPS) (PubMed:22532668). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10725698).;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sanadwich ELISA;Sensitivity: 0.039 ng/mL

Ly96 ORF Vector (Rat) (pORF)

ORF070129 1.0 ug DNA
EUR 607.2

Ly96 ORF Vector (Mouse) (pORF)

ORF049578 1.0 ug DNA
EUR 607.2

Ly96 ORF Vector (Mouse) (pORF)

ORF049579 1.0 ug DNA
EUR 607.2

Recombinant Lymphocyte Antigen 96 (LY96)

4-RPH705Hu01
  • EUR 528.58
  • EUR 265.20
  • EUR 1652.16
  • EUR 630.72
  • EUR 1141.44
  • EUR 429.60
  • EUR 3950.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Lymphocyte Antigen 96 expressed in: E.coli

Recombinant Lymphocyte Antigen 96 (LY96)

4-RPH705Mu01
  • EUR 571.58
  • EUR 276.00
  • EUR 1813.44
  • EUR 684.48
  • EUR 1248.96
  • EUR 458.40
  • EUR 4353.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Lymphocyte Antigen 96 expressed in: E.coli

LY96 Polyclonal Antibody, Biotin Conjugated

A59695-050 50 ul
EUR 302.5

Lasă un răspuns

Adresa ta de email nu va fi publicată. Câmpurile obligatorii sunt marcate cu *